Features
-
Animal-Derived Component Free
-
Protein-Free/Peptide-Free
-
Chemically Defined
-
DMF Completed
-
Universality: Supports the culture of various CHO cell lines (CHO-K1,CHOZN, HORIZON, CHO-S, CHO DG44, etc.) in fed-batch, high-density fed-batch and perfusion cultures
Advantages
Equivalent or superior performance to other media brands with better price and lead time
5 advanced CMPM (Cone Mixing/Pin Milling) powder media production lines to meet different customer demands with high inter-batch consistency (CPK*>1.33)
Safe and compliant: Chemically defined formulation minimizes the risk of contamination from animal-derived components and meets relevant compliance and regulatory requirements
EU certified ISO13485:2016 QMS and MDSAP (FDA), all data are traceable
Full documentation support available for IND application
“2 domestic + 1 imported†raw material supplier model to to ensure a stable and reliable supply chain
*CPK is a standard index to state the capability of one process.  CPK=1.33: the process is capable and meets specification limits. The higher the CPK, the better.
Order Information
Basal Medium
Fed-Batch Cultures
Application | Product Name | Cat. No. | Size | Form | Instructions (PDF) | |
CHO-K1, CHO-ZN, HORIZON, CHO-S, and CHO DG44, etc. | Eden B600S Basal Medium | EXP0109901 | 10 L | Powder | Download | |
EXP0109902 | 200 L | Powder | ||||
Eden B501S Basal Medium | EXP0106001 | 10 L | Powder | Download | ||
EXP0106002 | 200 L | Powder | ||||
Eden B401S Basal Medium | EXP0105901 | 10 L | Powder | Download | ||
EXP0105902 | 100 L | Powder | ||||
EXP0105903 | 200 L | Powder | ||||
Eden B300S Basal Medium | EXP0107901 | 100 L | Powder | Download | ||
EXP0107902 | 200 L | Powder | ||||
EXP0107903 | 10 L | Powder |
Application | Product Name | Cat. No. | Size | Form | Product Instruction (pdf) | |
CHO-K1, CHO-ZN, HORIZON, CHO-S, and CHO DG44, etc. | Eden F601aS Feed Medium | EXP0112101 | 20 L | Powder | Download | |
EXP0112102 | 10 L | Powder | ||||
EXP0112103 | 1 L | Powder | ||||
Eden F600aS Feed Medium | EXP0108901 | 20 L | Powder | Download | ||
EXP0108902 | 10 L | Powder | ||||
EXP0108903 | 1 L | Powder | ||||
Eden F500aS Feed Medium | EXP0105001 | 1 L | Powder | Download | ||
EXP0105002 | 20 L | Powder | ||||
Eden F400aS Feed Medium | EXP0104801 | 1 L | Powder | Download | ||
EXP0104802 | 20 L | Powder | ||||
Eden F600bS Feed Medium | EXP0108801 | 10 L | Powder | Download | ||
EXP0108802 | 1 L | Powder | ||||
Eden F200bS Feed Medium | EXP0104601 | 1 L | Powder | Download | ||
EXP0104602 | 10 L | Powder | ||||
EXP0104603 | 20 L | Powder | ||||
EXP0104604 | 50 L | Powder |
Performance
Antibodies
In fed-batch process, CHO cells cultured in Eden CD CHO media demonstrate higher viable cell density (VCD) and protein production than competitors. On average, the antibody titers of various CHO cell lines cultured in Eden CD CHO media ranged from 2 to 11 g/L.
In the pulse perfusion process, CHO cells cultured in Eden CD CHO media demonstrated superior performance than competitive global brands. When VVD=1.0, volumetric productivity (Vp) can reach up to 2.2 g/L/day, and the cumulative product expression in 14 days can reach 19 g/L, 55% higher than global brand B. When VVD=2.0, Vp can reach up to 3.3g/L/day, and the cumulative product expression in 14 days can reach 25 g/L.
Documents
CHO Cell Medium
FAQ
Q1: What is the packaging for BioEngine's powder media?
Q2:Can we seal the bag if there is leftover powdered medium?
Q3:What is the shelf life of BioEngine's Eden series CHO cell culture media, and how do you verify the expiration date?
Q4:Does BioEngine offer any regulators or additives for modulating antibody glycosylation?
Q5:Does BioEngine's CHO cell culture media contain hydrolysates, insulin, cytokines, or other components?
Q6: What are CHO media?
Q7: What are the differences between DMEM and RPMI?
1. Nutrient composition: DMEM (Dulbecco's Modified Eagle's Medium) and RPMI (Roswell Park Memorial Institute) have different nutrient compositions. DMEM contains higher levels of glucose, amino acids, vitamins, and sodium pyruvate, while RPMI has a lower glucose concentration and a different amino acid and vitamin composition.
2. pH: DMEM has a higher pH (7.4-7.6) compared to RPMI (7.2-7.4).
3. Usage: DMEM is a more general-purpose medium and can be used for a wide range of cell types, including adherent and non-adherent cells, while RPMI is typically used for the culture of immune cells such as lymphocytes and hybridomas.
4. Serum requirement: RPMI is often used with lower serum concentrations (e.g. 5-10%) than DMEM, which may require higher serum concentrations (e.g. 10-20%).
Overall, the choice between DMEM and RPMI depends on the specific cell type being cultured and the experimental conditions.
Q8: What is a fed-batch culture?
The goal of a fed-batch culture is to maximize cell growth and productivity while maintaining a stable culture environment. By controlling the rate and timing of nutrient addition, the culture can be kept in a state of controlled growth, avoiding the depletion of nutrients and accumulation of waste products that can limit growth and product formation in batch cultures. Additionally, the use of a fed-batch culture can allow for the accumulation of high cell densities and the optimization of production conditions, leading to higher yields and greater efficiency in bioprocesses.
Q9: What are the advantages of suspension cell cultures?
1. Scalability: Suspension cell cultures can be easily scaled up to produce large quantities of cells. This makes them particularly useful for biomanufacturing and the production of recombinant proteins.
2. Flexibility: Suspension cultures can be adapted to a wide range of culture conditions, such as pH, temperature, and nutrient availability. This allows for the optimization of cell growth and productivity.
3. Homogeneity: Suspension cultures provide a more homogeneous population of cells than adherent cultures, where cells may exhibit varying degrees of differentiation and proliferation.
4. Reduced risk of contamination: Suspension cultures are less prone to contamination by bacteria or fungi than adherent cultures, as there are no surfaces for microorganisms to adhere to.
5. Ease of harvesting: Cells in suspension culture can be easily harvested using centrifugation, filtration, or other methods. This simplifies downstream processing and reduces the risk of damage to the cells.
Overall, suspension cell culture offers several advantages over other types of cell culture, particularly in the context of large-scale biomanufacturing and the production of recombinant proteins.
Q10: What is gene therapy?
â—Replacing a disease-causing gene with a healthy copy of the gene.
â—Inactivating or deleting a disease-causing gene.
â—Introducing a new or modified gene to help fight disease.
Gene therapy is a promising and innovative field of medicine that has potential applications for many diseases, such as cancer, genetic disorders, infectious diseases, and autoimmune diseases 23. However, gene therapy also faces many challenges and risks, such as safety, efficacy, ethical issues, and regulatory hurdles.
Gene therapy usually be delivered to the cells by virus vector, such as AAV, Adv and RV. BioEngine provides vigor series insect cell media for large scale AAV production."
Q11: Does BioEngine provides serum products, like FBS?
Previous
No Information
Next
No Information
http://www.bioengine-global.com/